Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 31;8:11489. doi: 10.1038/s41598-018-29768-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

A comparison of 3D deconvolution software used to process a low SNR, 512 × 512 × 22 voxel widefield fluorescence focal stack. The focal stack was captured with three color channels (the first row) and then deconvolved with six deconvolution software methods (rows three through eight). The second row shows a confocal microscope image deconvolved with the Huygens software as a baseline for comparison. The fifth column shows the magnified image of the yellow rectangle in the corresponding mitochondria image. The composite images (the first column) show the sixth z slice of a pseudo-colored 3D image combining the three color channels. The nucleus, actin and mitochondria images (the second, third and fourth columns) show the eleventh, fourth and sixth z slice of the deconvolved 3D images. The scale bar in the composite image is 10 μm, and the scale bar in the magnified image is 3 μm. The number written in the figures are the minimum NMSE measured when comparing these data to deconvolved confocal images. All XY slices of the focal stacks are shown in a sequence in Supplementary Video 2.