Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 31;8:11489. doi: 10.1038/s41598-018-29768-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Deconvolution results for imaging of live cells expressing histone H2B fused to mClover to visualize chromosome conformation. (a) A focal stack (512 × 512 × 128 voxels) of the cell was captured over a span of one and a half hours with a time interval between focal stacks of three minutes. The 49-th z slice of the measurement, the deconvolved volume using our method, and the deconvolved volume using the Richardson-Lucy algorithm (100 iterations) is shown, with the time interval of fifteen minutes between frames along each row. (b) A second focal stack (512 × 512 × 100 voxels) shows a cell recorded for one hour with a time interval of three minutes. The 37-th z slice is shown, and the time interval between frames in each row is fifteen minutes. The corresponding deconvolved images are visualized in the second and third rows, respectively. The scale bar has the length of 10 μm. 3D volume renderings of these cells are provided as Supplementary Videos 3 and 4.