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. 2018 Jul 31;9:2992. doi: 10.1038/s41467-018-05385-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Jag1 deficiency results in reduced levels of ISC markers in the tumor cells. a qRT-PCR to measure the mRNA levels of selected ISC markers in wild-type and Jag1-deficient adenomas. The average and standard deviation (s.d.) of five tumors analyzed is represented. b In situ hybridization to determine the expression pattern of c-Myc and different stem cell markers in tumor sections from the indicated genotypes. c Quantification of the percentage of tumors containing detectable levels of the indicated genes as determined by ISH. A minimum of 20 tumors of each genotype was included in the analysis. d IF analysis of LGR5 protein in normal or adenoma tissue in the indicated genotypes and quantification of 15 randomly selected areas (right panels). e IHC analysis of the Paneth cell marker lysozyme and the absorptive marker carbonic anhydrase II (CAII) in tumors from the indicated genotypes. Graphs represent the number of tumors expressing these markers in the indicated genotypes. Unpaired t-test, two-tailed, was used to determine the statistical significance of the differences (***p < 0.001). Scale bars represent 75 μm