Table 1.

Dog characterization accordingly to gender, weight, age, breed, clinical classification, serological response to Leishmania, parasite isolation, and detection by quantitative polymerase chain reaction (qPCR).

Dogs	Gender	Weight (kg)	Age (months)	Breeds	Clinical classification (score)	Serology (DPP-LVC)®	Leishmania infantum isolation + qPCR [bone marrow (BM)]
Leish-Tec® (n = 12)	6 males	18–50	11–66	4 Shepherd Malinois	Low	T0 = 0/12	T0 = 0/12
	6 females			3 German Shepherds	(0) n = 12	T1 = 0/12
				4 Rottweilers		T6 = 1/12a
				1 Labrador		T12 = 0/12
CVL (n = 10)	7 males	5–15	24–192	8 mixed breeds	Low	Serology (DPP-LVC® and EIE-CL)	10/10
	3 females			1 Poodle	(1) n = 1	10/10
				1 Dachshund	Moderate
					(4) n = 1
					High
					(7) n = 2
					(8) n = 1
					(9) n = 4
					(11) n = 1
ACVL (n = 10)	7 males	5–25	12–84	9 mixed breeds	Low	10/10	9/10
	3 females			1 Labrador	(0) n = 9
					(1) n = 1

^aOne dog immunized with Leish-Tec^® presented positive DPP-LVC^® 6 months after vaccination although the EIE test was negative. Parasitological culture and qPCR were performed with BM and blood samples collected at the same time point and the results were negative. 1 year after immunization (T12), this dog tested negative in the DPP-LVC^®. All dogs from CVL and ACVL groups presented positive DPP-LVC^® and EIE tests.

Leish-Tec^®—vaccinated dogs, CVL – canine visceral leishmaniasis, ACVL – asymptomatic canine visceral leishmaniasis.