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. 2018 Jul 25;9:1713. doi: 10.3389/fimmu.2018.01713

Figure 4.

Figure 4

Effect of aryl hydrocarbon receptor (AhR) deletion on the recruitment of macrophages/microglia in experimental autoimmune uveitis (EAU) mice. (A) Left, representative immunofluorescent images of ionized calcium-binding adaptor molecule 1 (Iba1) (a marker of macrophage/microglia) and DAPI (nuclei) in retinas of AhR−/− and AhR+/+ EAU or non-immunized mice. Scale bar, 10 µm. Right, quantification of relative integrated optic density (IOD) of Iba1 (n = 4/group; mean ± SD;NS p > 0.05, ***p < 0.001; one-way ANOVA). (B) Left, representative Western blotting images of inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1), CD16 and CD206 in retinas of AhR−/− and AhR+/+ non-immunized mice. Right, quantifications of relative fold change of iNOS, Arg-1, CD16, and CD206 expressions (n = 4/group; mean ± SD;NS p > 0.05; unpaired Student’s t-test). (C) Left, representative Western blotting images of iNOS, Arg-1, CD16, and CD206 in retinas of AhR−/− and AhR+/+ EAU mice. Right, quantifications of relative fold change of iNOS, Arg-1, CD16, and CD206 expressions (n = 4/group; mean ± SD;* p < 0.05, **p < 0.01; ***p < 0.001; unpaired Student’s t-test).