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. 2018 Jul 25;9:1710. doi: 10.3389/fimmu.2018.01710

Figure 3.

Figure 3

Schistosoma mansoni Cathepsin B (Sm-Cathepsin B) + Montanide: parasite killing after the depletion of different cell populations. Sixty mechanically transformed schistosomula were incubated 24 h at 37°C, 5% CO2 under different conditions which included cells, cells + pre-immune serum, and cells + immune serum. Lung cells and serum were collected from the different immunized mouse groups: saline, Montanide ISA 720 VG, and Sm-Cathepsin B + Montanide ISA 720 VG. The different immune cell depletions were as follows: (A) parasite killing was analyzed for the control groups and the experimental group after CD4 cell depletion; saline n = 10, Montanide n = 10, Sm-Cathepsin B + Montanide n = 14. Furthermore, parasite killing was compared between Sm-Cathepsin B + Montanide CD45+CD4− and Sm-Cathepsin B + Montanide CD45+CD4+ (n = 14). (B) Parasite killing was analyzed for the control groups and the experimental group after CD8 cell depletion; n = 5 for all groups. Furthermore, parasite killing was compared between Sm-Cathepsin B + Montanide CD45+CD8− and Sm-Cathepsin B + Montanide CD45+CD8+ (n = 14). (C) Parasite killing was analyzed for the control groups and the experimental group after F4/80 cell depletion; saline n = 7, Montanide n = 7, Sm-Cathepsin B + Montanide n = 11. Furthermore, parasite killing was compared between Sm-Cathepsin B + Montanide CD45+F4/80− and Sm-Cathepsin B + Montanide CD45+F4/80+ (n = 14). (D) Parasite killing was analyzed for the control groups and the experimental group after CD49b cell depletion; saline n = 7, Montanide n = 7, Sm-Cathepsin B + Montanide n = 11. Furthermore, parasite killing was compared between Sm-Cathepsin B + Montanide CD45+CD49b− and Sm-Cathepsin B + Montanide CD45+CD49b+ (n = 14). ns: p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. The white columns represent data from the saline group post depletion. The gray columns represent data from the Montanide adjuvant group post depletion. The black columns represent data from the Sm-Cathepsin B + Montanide group post depletion. The single blue columns represent the undepleted Sm-CathepsinB + Montanide sample for the immune serum + cells culture condition.