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. 2018 Jan 4;596(15):3087–3100. doi: 10.1113/JP274833

Figure 1. ROS scavenger prevents LT‐IH‐induced ROS, AOE gene methylation and repression in the CB.

Figure 1

Top panel in A, rats were exposed to room air or exposed to LT‐IH and were treated with vehicle or MnTMPyP, a ROS scavenger during the first 10 days of IH exposure. A–C, analysis of malondialdehyde (MDA) levels (index of ROS levels; A), DNA methylation of AOE genes expressed as percentage of total cytosines that were methylated (methylated C; B), and AOE gene expression analysed by qRT‐PCR assay was normalized to 18S rRNA, and expressed as fold change from room air controls (C). Sod1, Sod2, superoxide dismutase 1 and 2; Cat, catalase; Txnrd2, thioredoxin reductase; Prdx4, peroxiredoxin 4; Gpx2, glutathione peroxidase 2. Data are presented as means ± SEM from 7 independent experiments for each treatment. ** P < 0.01; * P < 0.05; ns, not significant, P > 0.05.