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. 2018 Jan 4;596(15):3087–3100. doi: 10.1113/JP274833

Figure 5. Carotid body ablation (CBA) prevents LT‐IH‐induced ROS, DNA methylation and repression of AOE genes in the AM.

Figure 5

AMs were harvested from sham‐operated rats exposed to room air; sham‐operated rats exposed to 30 days of IH with intact CB (LT‐IH); and CB‐ablated rats exposed to 30 days of IH (LT‐IH + CBA). A, aconitase activity in cytosolic and membrane fractions (index of ROS). B, DNA methylation of AOE genes expressed as percentage of total cytosines that were methylated (methylated C). C, AOE gene expression analysed by qRT‐PCR assay was normalized to 18S rRNA, and expressed as fold change from room air controls. Sod1, Sod2, superoxide dismutase 1 and 2; Cat, catalase; Txnrd2, thioredoxin reductase; Prdx4, peroxiredoxin 4; Gpx2, glutathione peroxidase 2. Data presented as means ± SEM, n = 6 rats per group; * P < 0.05; ns, not significant, P > 0.05.