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. 2018 Jan 4;596(15):3087–3100. doi: 10.1113/JP274833

Figure 6. Effect of ROS scavenger on Dnmt enzyme activity and proteins in AM of LT‐IH exposed rats.

Figure 6

AMs were harvested from rats exposed to room air or exposed to LT‐IH and were treated with either vehicle (LT‐IH + vehicle) or MnTmPyP (LT‐IH + MnTmPyP), a ROS scavenger during the first 10 days of IH exposure. A, Dnmt enzyme activity. B, representative immunoblots of Dnmt1, Dnmt3a, Dnmt3b, and TATA binding protein (TBP) as loading control. C, quantitation by densitometric analysis of Dnmt proteins. Molecular weights of proteins are presented as kilodaltons (kDa). Data in bar graphs are presented as means ± SEM from 5 independent experiments. * P < 0.05; ns, not significant, P > 0.05 compared to room air exposed control rats.