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. 2018 Jul 25;9:800. doi: 10.3389/fphar.2018.00800

FIGURE 4.

FIGURE 4

Effects of A11 and A25 on RANKL-induced osteoclastogenesis. (A) TRAP staining of osteoclastogenic cultures. BMMs were treated with A11 and A25 (1, 2, 5, and 10 μM) in the presence of RANKL (50 ng/ml) and M-CSF (25 ng/ml) for 5 days. (B) BMMs were treated with A11 (1, 2, 3, 4, 5, 7.5, and 10 μM) and A25 (1, 5, 10, and 20 μM) in the presence of RANKL (50 ng/ml) and M-CSF (25 ng/ml) for 4 days and cell lysates were measured for TRAP activity. % TRAP activity per microgram of total protein was expressed as a percentage of the untreated control. IC50 values are given as mean ± SEM from three independent experiments performed in duplicate.