Table 1. Primary antibodies and protocols for immunohistochemistry.

Antibody	Host (Clone)	Dilution	Pre-treatment	Manufacturer	Normal mesothelial cell	Tumor cell
Cytokeratin	Mouse (AE1/AE3)	RTU	pH 6.0 AC	Dako (Tokyo, Japan)	+	+
Vimentin	Mouse (V9)	RTU	pH 6.0 AC	Dako (Tokyo, Japan)	+	+
CEA	Rabbit	1:400	pH 6.0 AC	Dako (Tokyo, Japan)	-	-
Mesothelial cells	Mouse (HBME-1)	1:50	None	Dako (Tokyo, Japan)	+	-
WT-1	Rabbit	1:100	pH 6.0 AC	Acris (Herford, Germany)	+	+

CEA, carcinoembryonic antigen; WT-1, Wilms tumor-1; RTU, ready-to-use; AC, autoclaved; +, positive; -, negative.