Skip to main content
. 2018 Jun 14;12(8):1324–1341. doi: 10.1002/1878-0261.12328

Figure 6.

Figure 6

miR‐106a and LITAF regulate ATM expression to confer radioresistance. (A) qRT‐PCR for ATM mRNA expression (normalized to GAPDH) in PC3 and DU145 control(m)/miR‐106a(106a) mimic and control(si)/LITAF siRNA cells. (B) Representative western blot for phospho‐ATM (pATM), total ATM, and β‐actin (loading control) in PC3 control/miR‐106a mimic and control/LITAF siRNA cells at 0 Gy and 30 min after 6 Gy irradiation (IR). (C) ATM promoter luciferase in PC3 and DU145 control/miR‐106a mimic cells. (D) Representative western blot for pATM, total ATM, and β‐actin from PC3 cells were treated with DMSO only (vehicle), 1, 2, 5, and 10 μm of KU‐55933 2 hr before exposure to 6 Gy IR, and baseline at 0 Gy with DMSO only. (E) SA‐β‐galactosidase assay was performed with DU145 control/miR‐106a cells 7d after 6 Gy IR. Cells were treated with DMSO and 2 μm KU‐55933. Representative images show DU145 control/miR‐106a cells after 6 Gy stained for SA‐β‐galactosidase with DMSO or KU‐55933 (scale bar = 200 μm). (F) Clonogenic survival assays were performed with DU145 cells transfected with control and miR‐106a mimic. PC3 and DU145 cells were treated with DMSO or 2 μm KU‐55933 (KU) ATM inhibitor 2 h prior to 6 Gy or 8 Gy radiation, respectively. Mean, SEM, and statistical significance are denoted; *, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 3 biological replicates.