Table 3.

Plasmid conjugation studies.

		Disk diffusion test*		PCR
Strains	MIC to colistin (μg/mL)	NAL	CIP	mcr-1/PMQR/CTX-M
E. coli 190-14	8	R	R	mcr-1+ qnrB+qnrS+CTX-M-2
EC190-14 TC**	4	I	S	mcr-1+ qnrB
E. coli 191-07	8	I	S	mcr-1+ qnrB+ CTX-M-2+ CMY-2
EC191-07 TC**	4	I	S	mcr-1+ qnrB
E. coli J53	0,5	S	S	–
EC191-07 TCS***	8	I	S	mcr-1+ qnrB
Salmonella M1744	1	S	–	–

^*(R) resistant, (I) intermediate and (S) susceptible by disk diffusion test: Nalidixic Acid (NAL), Ciprofloxacin (CIP).

^**TC: transconjugants obtained using E. coli J53 as the recipient strain.

^***TCS: transconjugants obtained using Salmonella M1744 as the recipient strain.