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. 2018 Jul 31;9(4):e01401-18. doi: 10.1128/mBio.01401-18

FIG 4  .

FIG 4 

MAb 6E4 Western blot with proteinase K extracts of NTHi strains (wild type and mutants). Wild-type strain 2019 (lanes 1 and 2), ΔsiaA mutant (lanes 3 and 4), Δlic3 mutant (lanes 5 and 6), Δlic3B mutant (lanes 7 and 8), ΔlsgB mutant (lanes 9 and 10), ΔsiaA Δlic3 Δlic3B mutant (lanes 11 and 12), ΔsiaA Δlic3A ΔlsgB mutant (lanes 13 and 14), ΔsiaA Δlic3B ΔlsgB mutant (lanes 15 and 16), and ΔsiaB Δlic3A ΔlsgB mutant (lanes 17 and 18) were tested. Extracts in the even-numbered rows were from strains grown in the presence (+) of 100 μM Neu5Ac. As can be seen, MAb 6E4 did not react with any extracts derived from strains grown in the presence of 100 μM Neu5Ac, and reactivity with MAb 6E4 occurred only when the LsgB sialyltransferase was active in NTHi strain 2019. The slight band in lane 12 is due to spillover from lane 11.