FIG 5  .

(A) lsgB expression in strain 2019 background when the organism is grown on an agarose surface (bar A) or in broth (bar B). As can be seen, there is a significant approximately fourfold increase in expression of lsgB when NTHi strain 2019 is grown on solid medium (P < 0.0001). (B) No significant differences in lsgB expression in NTHi 2019siaR strain comparing growth on solid medium (bar A) to growth in broth (bar B). The lack of difference in expression of the lsgB gene under these different growth conditions suggests that SiaR may play a role in the regulation of lsgB expression. All studies were performed in triplicate and measured in triplicate in the assay.