Expression, translation, and regulated evidence of de novo genes. (A) Dynamic expression of six de novo genes under different conditions. The FPKM values are in the range of 0 to ~26. All expression data of de novo genes are shown in Table S1. (B) Dynamic translation of six de novo genes under different conditions. The FPKM values are in the range of 0 to ~10. All footprinting data of de novo genes are shown in Table S4. (C) Various regulations of de novo genes associated with nutrient utilization, developmental stages, and cell aging. Red represents fixed de novo genes, and gray represents the segregating de novo genes. All regulation data of de novo genes are shown in Table S3.