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Comparison of de novo and conserved genes by (A) gene length, (B) GC content, and (C) gene expression level. For the intergenic regions, 4,539 fragments ranging from 200 to 1,000 nucleotides (nt) were used. The conserved genes were defined such that these genes are shared among S. cerevisiae S288C, S. paradoxus CBS432, and S. mikatae IFO1815.
