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. 2018 Jul 31;92(16):e00905-18. doi: 10.1128/JVI.00905-18

FIG 1.

FIG 1

TRIM41 colocalizes and interacts with NP. (A) TRIM41-HA (1.25 μg) was cotransfected with 1.25 μg of FLAG-tagged NP from the IAV PR8 or NY strain into HEK293 cells. Cell lysates were immunoprecipitated (IP) with anti-FLAG antibody or control IgG and then blotted as indicated. Molecular weights are indicated. (B) A549 cells were infected at an MOI of 1 with PR8 IAV for 12 h. Cell lysates were treated with or without RNase A and then immunoprecipitated with anti-NP antibody or control IgG. (C) A549 cells were mock infected or infected with PR8 IAV. After 12 h, cells were fixed with cold methanol and incubated with anti-NP and anti-TRIM41 antibodies. DAPI, 4′,6′-diamidino-2-phenylindole. (D) The schematics of TRIM41 mutants. RING, really interesting new gene; B-Box, B-box type zinc finger; CC, coiled coil; SPRY, SPla and the RYanodine receptor. (E) HA-tagged NP (NP-HA; 1.25 μg) was cotransfected with 1.25 μg of the indicated FLAG-tagged TRIM41 mutants into HEK293 cells. Cell lysates were immunoprecipitated with anti-FLAG antibody and blotted with the indicated antibodies. WB, Western blotting.