FIG 2.

TRIM41 expression is not modulated by IFN and IAV. (A) A549 cells were treated with the designated units (U) of IFN-β for 12 h. Cell lysates were blotted with the indicated antibodies. The interferon-induced transmembrane protein 3 (IFITM3) was included as a positive control. (B) A549 cells were infected at the indicated MOI with PR8 ΔNS1 IAV for 12 h. Cell lysates were blotted with the indicated antibodies. IFITM3 was included as a positive control. (C) A549 cells were infected at the indicated MOI with the ΔNS1 mutant virus for 4 h. mRNA was extracted, and real-time PCR was performed to determine the relative levels of the indicated genes. IFN-β and IFN-γ-inducible protein 10 (IP10) are the positive-control genes that are known to be induced by viral infection. (D) A549 cells were treated with 1 unit of IFN-β for the designated times. mRNA was extracted, and real-time PCR was performed to determine the relative levels of the indicated genes. All experiments were biologically repeated three times. RANTES (regulated on activation, normal T cell expressed and secreted) and IP10 were the positive controls.