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Detection of cross-reactivity by gamma interferon ELISpot assay. The CTL clone S00001-SL9-3.23 recognizing the A*02-restricted minimal epitope SLYNTVATL (Gag 77–85:, SL9) was screened against seven other known A*02-restricted HIV-1 minimal epitopes (Table 2) in a standard ELISpot assay. Background-subtracted counts are plotted. The data are representative of two independent experiments.
