FIG 1.
Experimental design. Twenty-three Mamu-B*17+ RMs were vaccinated with an rYF17D/EP rDNA/rAd5/rVSV/rRRV regimen and divided into three groups depending on which vaccine inserts they received. Animals in group 1 (n = 7) were vaccinated with vif and nef sequences. Animals in group 2 (n = 8) were vaccinated with the same SIV inserts plus env. RMs in group 3 were sham vaccinated with vectors lacking SIV genes or expressing irrelevant inserts and served as the control group for this experiment. At study week 101, vaccine efficacy was assessed by subjecting all animals to repeated intrarectal (i.r.) challenges with a marginal dose of SIVmac239 (200 TCID50) every 2 weeks.