FIG 2.

Development of vaccine-induced CD8⁺ T-cell responses against Mamu-B*17-restricted SIV epitopes in groups 1 and 2. Fluorochrome-labeled Mamu-B*17 tetramers folded with peptides corresponding to SIV epitopes were used to track vaccine-elicited SIV-specific CD8⁺ T cells in PBMC from RMs in group 1 (A, C, and E) and group 2 (B, D, F, and G). The percentages of live tetramer⁺ CD8⁺ T cells specific for Vif HW8 (A and B), Nef IW9 (C and D), Nef MW9 (E and F), and Env FW9 (G) are shown at multiple time points throughout the vaccine phase. No data for Env FW9-specific CD8⁺ T cells are available for the group 1 RMs because those animals were not vaccinated with env. The times of each vaccination (black vertical dotted lines) and the day of the first i.r. SIV challenge (red vertical solid line) are indicated in each graph. RMs in groups 1 and 2 are color-coded in blue and black, respectively.