FIG 2.

HPV-18 E6 is phosphorylated as a result of cellular stress responses. (A) Representative Western blot analysis of HeLa cells either untreated (UN) or treated with H₂O₂ for 4 h, nocodazole for 15 h, or CHX for 4 h in the presence or absence of the antioxidant NAC. The blots were probed with antibodies against total HPV-18 E6 and HPV-18 E6 phospho-T156 (18-pE6); α-actinin was used as a loading control. (B) Relative quantification of Western blots was done using Image J software, where the ratio of the net intensity of pE6 to the net actinin loading control was calculated. The histogram shows the results from at least three independent experiments, statistically quantified by Student's t test; the error bars indicate standard errors of the mean. *, P = 0.05; ns, nonsignificant.