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. 2018 Jul 31;92(16):e00001-18. doi: 10.1128/JVI.00001-18

FIG 10.

FIG 10

HIV-1 DNA-triggered signaling is not rapid enough to impair the initial ISG-triggering infection cycle. The THP-1 cells, THP-1 cGAS KO, and THP-1 DKO cells were infected with luciferase- or GFP-encoding single-cycle viruses at a range of MOIs. Samples were assayed for ISRE and viral luciferase activities or analyzed for percent positive GFP by fluorescence-activated cell sorting analysis at 48 hpi. (A and B) ISRE activity and viral luciferase activity were determined at 48 hpi in THP-1, THP-1 cGAS KO, and THP-1 DKO clone 15, and THP-1 DKO clone 26 cells infected with HIV (LAI) luciferase reporter virus. (C and D) ISRE activity and percent positive GFP cells were determined at 48 hpi in THP-1, THP-1 cGAS KO, THP-1 DKO clone 15, and THP-1 DKO clone 26, and THP-1 TREX1KO-11 cells infected with HIV-1GFP reporter virus. The results represent means and standard deviations of biological triplicates from two independent experiments.