FIG 5.

Agenet domains have an incomplete aromatic cage. Multiple-sequence alignments comparing the Agenet domains of EML1-4, three Arabidopsis Tudor/PWWP/MBT superfamily domain proteins (AT1G80810, AT4G31880, and AT5G10950) sharing a high percentage of amino acid sequence similarity with EML Agenet domains, and the Tudor domains of the structural animal homologs PHF1 and PHF19 were constructed using ClustalW2. Highlighted residues in PHF1 and PHF19 are characterized, conserved aromatic residues necessary for the formation of the aromatic cage for H3K36me3 binding. Asterisks mark identical residues. Dots mark similar residues with various degrees of conservation.