FIG 1.
Decreased Ulk1 expression in the CNS of PHEV-infected mice. (A) Mice that were intranasally inoculated with 100 μl PHEV (104.45 TCID50/0.1 ml) showed typical neurological symptoms at 3 dpi, such as abnormal gait, tremors, listlessness, standing upright, raised forelimbs, and arched waists. Mice in the control group survived normally. (B) Survival curves of PHEV-infected mice (n = 10 mice per group; data from three independent experiments). (C) Body weight curves of PHEV-infected and control mice from 0 to 6 dpi. From 4 dpi, PHEV-infected mice exhibited a significantly lesser body weight than that observed in the control mice. (D) qRT-PCR analysis of Ulk1 mRNA levels in mouse brain after PHEV infection. Data are presented as the means ± standard errors of the means (SEM) (n = 6). (E) In situ hybridization of the mouse brain for Ulk1 mRNA (green) with an antisense probe. Quantitative analysis is summarized in the histograms on the right. The y axis is the average Ulk1 mRNA intensity in arbitrary fluorescence units.