TABLE 2.
Virus properties in DBA/2 mice
| Recombinant virus | Rescue frequency | Survival | Max % wt lossa | Lung titerb |
|---|---|---|---|---|
| WT | 2/2 | 4/10 | 21.7 ± 7.2 | 5.1 ± 0.1 |
| rTN09PANluc | 2/2 | 5/5 | 6.7 ± 3.4 | 4.1 ± 0.3 |
| rPB2-E158G | 0/10 | NDc | ND | ND |
| rPA-D479N | 2/2 | 5/5 | 8.1 ± 4.5 | 4.4 ± 0.3 |
| rPB2-E158G/PA-D479N | 1/4 | 5/5 | 10.2 ± 5.4 | 4.3 ± 0.2 |
| rPB2-MA9 | 2/2 | 2/10 | 23.5 ± 8.3 | 4.8 ± 0.1 |
| rPB2-MA9/PA-D479N | 2/2 | 0/10 | 26.7 ± 1.3 | 5.4 ± 0.1 |
a
The maximum change in the mean value (percentage) ± standard deviation observed over a period of 14 days postinoculation.
b
Lung titer (log10 PFU/ml) was determined 4 days postinoculation.
c
ND, not determined because virus was not rescued by reverse genetics.