TABLE 3.
Nluc reporter gene stability of rPB2-MA9/PA-D479N after passage
Mouse | Retention of Nluc activity (no. of plaques/total) |
||
---|---|---|---|
C1M1a | C6M1b | C1A5M1c | |
1 | 9/9 | 10/10 | 10/10 |
2 | 10/10 | 10/10 | 10/10 |
3 | 10/10 | 10/10 | 10/10 |
4 | 10/10 | 10/10 | 10/10 |
5 | 10/10 | 10/10 | 10/10 |
One passage in MDCK cells and 4 days of infection in DBA/2 mice.
Six passages in MDCK cells and 4 days of infection in DBA/2 mice.
One passage in MDCK cells, 5 passages in A549 cells, and 4 days of infection in DBA/2 mice.