TABLE 2.

Quantification of tracks

Microscopy type and track no.a	Start time (s)	End time (s)	Duration (s)	Total displacement (μm)b	Net displacement (μm)c	Peak velocity (μm/s)d
iSIM
1	0	8	8	14.15	9.77	4.7
2	0	3	3	3.96	1.67	3.1
3	0	13	13	7.82	2.71	1.4
4	0	8	8	12.92	10.58	5.9
5	0	9	9	9.23	2.23	3.8
6	0	9	9	8.80	0.69	3.3
7	0	18	18	3.09	2.1	0.8
8	0	14	14	9.96	5.71	2.8
9	0	13	13	10.45	1.37	2.8
10	0	7	7	5.9	4.65	2.3
TIRF
0	0	84	84	23.76	3.54	1.2
1	85	120	35	11.58	10.23	1.5
2	83	119	36	4.58	3.62	0.6
3	81	110	30	14.07	12.77	2.3
4	77	88	12	11.05	9.09	1.8
5	23	47	24	11.41	2.33	2.4
6	0	120	120	42.83	6.25	2.5
7	14	26	12	9.76	6.78	1.6
8	103	120	17	4.23	3.55	0.8
9	1	24	23	5.06	3.22	1.1
10	5	120	115	27.12	2.42	0.8

^aiSIM, instant structured illumination microscopy; TIRF, total internal reflection fluorescence microscopy.

^bTotal displacement, the sum of all consecutive displacements in each track, which corresponds to the total distance traveled by the object.

^cNet displacement, the distance between the starting and ending positions of each track.

^dPeak velocity, highest velocity of the object over the duration of each track.