FIG 4.

Cytokine response to BOR-57 PRRSV infection. Cytokine levels in serum samples collected prior to challenge on day 0 and on challenge days 3, 7, 10, and 14 were measured using cytokine antibody arrays. (A) Alpha interferon (IFN-α); (B) interleukin-17A (IL-17A); (C) interleukin-1 receptor antagonist (IL-1ra); (D) IL-4; (E) IL-6; (F) IL-8; (G) monokine induced by gamma interferon (MIG/CXCL9); (H) macrophage inflammatory protein 1β (MIP-1β/CCL4); (I) chemokine ligand 3-like 1 (CCL3L1); (J) granulocyte-macrophage colony-stimulating factor (GM-CSF); (K) tumor necrosis factor alpha (TNF-α); (L) IL-12; (M) IL-1β; (N) IL-10; (O) transforming growth factor β1 (TGFβ1); (P) IFN-γ; (Q) IL-18; (R) platelet endothelial cell adhesion molecule 1 (PECAM-1/CD31); (S) IL-1α; (T) IL-13. Error bars represent SEM (duplicates of 4 replicates). Statistical analysis was performed using two-way ANOVA and Sidak's multiple-comparison test. *, P ≤ 0.5; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.