Table 3.

Cycling parameters for polymerase chain reaction programs of selected probiotics.

Cycling Parameters *	PCR Program for Lactobacillus Genus	PCR Program for Bifidobacterium Genus	Multiplex Program for L. rhamnosus, L. acidophilus, B. bifidum	Duplex Program for L. casei and L. reuteri	PCR Program for B. coagulans	PCR Program for E. faecium	PCR Program for E. coli
Denaturation	30 s at 94 °C	30 s at 94 °C	45 s at 94 °C	30 s at 94 °C	30 s at 94 °C	60 s at 94 °C	30 s at 94 °C
Annealing	30 s at 55 °C	60 s at 53 °C	45 s at 63 °C	30 s at 55 °C	30 s at 60 °C	60 s at 54 °C	30 s at 52 °C
Extension	30 s at 72 °C	2 min at 72 °C	60 s at 72 °C	30 s at 72 °C	60 s at 72 °C	60 s at 72 °C	60 s at 72 °C
Number of cycles	20	35	30	30	30	40	35

* Initial denaturation and final extension is 15 min at 95 °C and 7 min at 72 °C respectively for all amplifications.