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Effects of Klotho on Ang II-induced proliferation of VSMCs. (A) This was observed via CCK-8 assay. VSMCs were pretreated in serum-free medium in the presence or absence of Klotho (25, 50, 100, 200 ng/ml) for 24 h, and then stimulated with 10−7 M Ang II for a further 24 h. (B) VSMCs were treated with 200 ng/ml Klotho for 24h and then treated with Ang II for different time (6 h, 12 h, 24 h, 48 h). # p<0.05 versus untreated cells. * p<0.05 versus Ang II treatment.
