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. 2018 Jul 13;24:4851–4860. doi: 10.12659/MSM.908038

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© Med Sci Monit, 2018

This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

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Effects of Klotho on Ang II-induced migration of VSMC. (A) Wound healing assay. Conflunet VSMCs were scapes wounded and allowed to migrate for 24 h. (B) Transwell chemotactic assay. Klotho inhibited Ang II-induced VSMCs miagration. (C) Cell morphology and actin filaments analysis by laser confocal microscopy. VSMCs were incubated with Alexa 546-conjugated rhodamine phalloidin (red) and the nuclei were stained with DAPI (blue). * p<0.05 versus untreated cells. ^# p<0.05 versus Ang II treatmen.