Figure 4.

Effects of Klotho on Ang II induced cell cycle progression in VSMCs. Cell cycle progression was assessed by flow cytometric analysis of DNA content. The cells were pretreated in the presence or absence of Klotho in serum-free medium for 24 h and then stimulated with 10⁻⁷ M Ang II. After 24 h, individual nuclear DNA content is reflected by the fluorescence intensity of incorporated propidium iodide. Each item is derived from representative experiments, where data from at least 10,000 events were obtained.