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Effects of Klotho on Ang II-induced cell cycle related-protein in VSMCs. Cell were pretreated in a serum-free medium in the presence or absence of Klotho for 24 h. The cells were lysed, and proteins were analyzed by Western Blot. GAPDH was used as an internal control. The graphs represent the relative expression of these proteins for three independent experiments. * p<0.05 versus untreated cells. # p<0.05 versus Ang II treatment.
