Extended Data Figure 10 |. WNT/β-catenin pathway expression correlates with responsiveness to I-BET in primary human AML samples.

a, Assessment of β-catenin pathway gene expression in eight primary human AML samples with associated response to I-BET exposure. Each panel represents an individual primary human AML sample, with genetic abnormality denoted. Waterfall plot of relative qRT–PCR expression data of key β-catenin pathway genes (AXIN2, CCND1, CTNNB1, FZD5, MYC, TCF4 (also known as E2-2)) is displayed. Each bar is labelled 1–6 according to gene represented. Relative apoptosis observed after 48 h exposure to 500 nM I-BET versus vehicle (0.1% DMSO) is denoted in square parenthesis and is also represented as a heat map background shading in each panel. b, log₂-transformed expression levels of selected genes in the WNT/β-catenin pathway were measured using qRT–PCR. A corrgram shows the genes are highly correlated with each other. The colour and thinness of the ellipse indicate the strength of correlation (a line is perfect correlation; a circle is uncorrelated). The ellipse direction indicates the sign of the correlation (correlated: right/blue, inversely correlated: left/red). c, Expression of selected genes is correlated with apoptosis. Scatterplots show apoptosis versus the log₂ expression level of each gene. Expression of five genes (CCND1, CTNNB1, FZD5, MYC and TCF4) predicts apoptosis. The relationship is highlighted in a plot of apoptosis predicted using a multiple linear regression model with the five genes versus the actual data. d, Apoptosis observed after 48 h exposure to either vehicle (0.1% DMSO) or 500 nM I-BET across eight primary human AML samples. e, Relative viability of primary human AML samples after treatment with I-BET.