Fig. 3.

Activation of the RISK pathway by IP and NRG-1 in vivo. (a), Representative protein levels of P-ERK1/2 and T-ERK1/2 by western blotting. (b), Semi-quantification of the density ratio of P-ERK1/2/T-ERK1/2. (c), Representative protein levels of P-AKT and T-AKT by western blotting. (d), Semi-quantification of the density ratio of P-AKT/T-AKT. (e), Representative protein levels of P-AMPK and T-AMPK by western blotting. (f), Semi-quantification of the density ratio of P-AMPK/T-AMPK. These protein levels were normalised to GAPDH. CON: control, IR: ischaemia-reperfusion, IP: ischaemic postconditioning, NRG-1: IR + NRG-1. Data are shown as the mean ± SEM (n = 6). #p < 0.05 vs. CON; *p < 0.05, **p < 0.01 vs. IR