Table 2.

Primary and secondary antibodies for immunofluorescence staining

Antibodies	Dilution	Manufacturer
I Ab: Mouse-anti-human ZO-1	1:250	Life Technologies, Carlsbad, USA
I Ab: Ki67	1:500	Millipore, Darmstadt, Germany
I Ab: Laminin	1:100	Abcam, Cambridge, UK
I Ab: ColI	1:250	Abcam, Cambridge, UK
II Ab: Alexa Fluor 488 donkey-anti-rabbit	1:800	Molecular Probes, Leicestershire, UK
II Ab: Alexa Fluor 568 donkey-anti-mouse	1:800	Molecular Probes, Leicestershire, UK

I Ab primary antibody, II Ab secondary antibody