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. 2018 Jul 31;17:110. doi: 10.1186/s12943-018-0860-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Production of CYTOR mutant cells by CRISPR/Cas9. a Prediction of secondary RNA structure of wild-type (WT) CYTOR and various exon-deleted CYTOR mutants. b Schematic diagram for CRISPR/Cas9 and donor vector to delete EXON1 or EXON4 of CYTOR. c RT-PCR and gel electrophoresis to assess the editing efficiency of CRISPR-sgRNA specific to EXON 1. *indicates Non-homologous end joining with an Indel. d RT-PCR and gel electrophoresis to assess the editing efficiency of CRISPR-sgRNA specific to EXON 4. *indicates Non-homologous end joining with an Indel. e DNA sequencing to identify the exon1-deleted RKO cells (ΔEXON1) through clone screening. f DNA sequencing to identify the exon4-deleted RKO cells (ΔEXON4) through clone screening