Fig. 3.

Cell shape changes in the midline after mid-hindbrain neuropore (MHNP) closure in vivo. a The relationship between the closing of MHNP and the somite stage in ICR strain mouse. b-f Dorsal views of embryos stained with E-cadherin (Ecad). Non-neural ectodermal cells change their shapes dramatically from bipolar to polygonal after the MHNP closure. g, h Higher manigification views of embryos (c, e) that were co-stained with Ecad, apoptotic marker active-Caspase 3 (aCasp 3), and nuclear staining (DNA). Dorsal views, left is rostral side. Scale bars: (b-f) 100 μm in upper panels, and 25 μm in (g, h) middle and (b-f) lower panels