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. Author manuscript; available in PMC: 2019 Mar 16.
Published in final edited form as: J Am Chem Soc. 2018 May 3;140(19):6109–6121. doi: 10.1021/jacs.8b02279

Figure 2.

Figure 2.

(a) Confocal fluorescence microscopic images of HeLa cells treated with Peroxymycin-1 (1 μM) for indicated time intervals. The cells were then washed, fixed, stained and imaged. (b) Confocal fluorescence microscopic images of HeLa cells treated with PF2 (10 μM) for 0.5, 4, and 6 h, respectively. The medium was then replaced by phosphate-buffered saline (PBS) solution and imaged. All images in panels a and b were recorded by use of the same imaging parameters with the Alexa488 channel. (c) Normalized cellular fluorescence intensities of the HeLa cells as determined by ImageJ, showing superior sensitivity for H2O2 detection for the new histochemical boronate H2O2 probe over a fluorescent probe counterpart. Error bars denote standard derivation (SD; n = 5).