Figure 4.
Confocal fluorescence microscopic images of HeLa cells stained with (a) Peroxymycin-1 (1 μM) or (b) puromycin (1 μM) for 6 h, with or without coincubation of paraquat (1 mM) for the indicated time intervals. The cells were subsequently washed, fixed, stained, and imaged. All images were recorded by use of the same imaging parameters with the Alexa488 channel. (c) Cellular fluorescence intensities of HeLa cells as determined by ImageJ. Error bars denote SD (n = 5). *p < 0.05 and **p < 0.01.