Figure 2.

SK-treated 4T1-luc2 cells effectively immunized mice against primary tumors. Test mice (n = 10) were vaccinated with varying dosages (cell numbers) of F/T treated 4T1-luc2 cells or test cells treated with 5 μM SK for 24 h. At 7 d post-vaccination, live 4T1-luc2 tumor cells were implanted. (A) Tumor growth rate. Tumor volume was monitored until 37 d post tumor implantation. (B) Tumor-free incidence and (C) mouse survival rates were recorded until 70 d post tumor implantation. (D) Tumor progression in test mice visualized by bioluminescence imaging (BLI). BLI was determined at 28 d post tumor implantation. Fluorescence images are shown to present data on tumor growth.