Figure 8.

SETD7 overexpression significantly increased H/R-induced apoptosis in cardiomyocytes. (Ai and ii) The indicated plasmids were transfected into H9c2 cells and then exposed to H/R. Apoptosis was measured by ANXA5-PI staining. (Aiii) Apoptotic enzymes CASP3 and CASP7 activity was measured for the indicated H9c2 cells. The data from 3 independent experiments are presented as the mean ± SD. (B and C) Western blotting and immunoprecipitation assays were respectively used to indicate that a potent and selective inhibitor of SETD7, (R)-PFI-2 (1 μM, 18 h), inhibited LC3B-II expression and ATG16L1 methylation at K151.