FIG. 1.

BDE-47 and BDE-49 do not alter dendritic growth in cultured hippocampal neurons. Cells dissociated from P1 rat hippocampi were transfected with MAP2B-eGFP at DIV 6. On DIV 7, cultures were exposed to vehicle (DMSO diluted 1:1000) or varying concentrations of BDE-47 or BDE-49 for 48 h. A, Representative photomicrographs of DIV 9 neurons expressing MAP2B-eGFP following exposure to vehicle, BDE-47 (200 nM) or BDE-49 (200 nM). PCB 95 (200 nM) was added to a subset of cultures as a positive control. B, Quantification of dendritic length in GFP+ neurons. Data from one experiment presented as the mean ± SE (n = 30–40 neurons per condition). Experiments were repeated in 3 independent dissections with comparable results from each experiment. *P < .05 relative to vehicle control. Scale bar = 25 µm.