FIG. 5.

BDE-47 and BDE-49 delay the development of polarity in hippocampal neurons. Polarity was quantified in dissociated hippocampal cell cultures based on the subcellular distribution of GAP-43 immunoreactivity and morphometric criteria. A, Representative photomicrographs of hippocampal neurons at different stages of polarization. B, Ontogeny of polarity in hippocampal cultures grown under the culture conditions used in this study. C, Percent of polarized (Stage 3) neurons at DIV 2 following a 48 h exposure to vehicle, BDE-47 (200 nM) or BDE-49 (200 nM). Data presented as mean ± SE (n = 9 coverslips collected from 3 independent dissections, 70–150 neurons each coverslip). *P < .05 relative to vehicle control. Scale bar = 10 µm.