FIG. 7.

BDE-47 (200 nM) and BDE-49 (200 nM) reduce axon length via RyR2-dependent mechanism(s). A, Representative photomicrographs of axonal growth cones in DIV 2 hippocampal cultures co-labeled with fluorescently tagged phalloidin (green) and antibody selective for RyR1 (red, left) or RyR2 (red, right). B, Quantitative analyses of axonal length in Cy5 positive neurons. Dissociated hippocampal cells were electroporated with Cy5-labeled scrambled (control), RYR1 or RYR2 siRNA prior to plating. Data presented as the mean ± SE (n = 90 neurons collected from 3 independent dissections). *P < .05 relative to vehicle control, #P < .05 relative to cultures transfected with control siRNA and exposed to the same BDE. Scale bar = 5 µm.