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Localization of mature miR172s and cly1 by RNA in situ hybridization in the immature spike sampled at the awn primordium stage. Plant genotypes were AZ (Cly1.a) (A–D) and KNG (cly1.b) (E–H). Spike RNAs were hybridized with miR172a probe (A, E), miR172b probe (B, F), scrambled miRNA probe (negative control) (C, G) and cly1 probe (D, H). Cs, central spikelet; Ls, lateral spikelet; Lo, lodicule; Ra, rachis; Ca, carpel; St, stamen. Scale bars = 0.1 mm.
