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Effect of absence of miR172a on accumulation of miR172 and cly1 transcripts in the immature spike sampled at the awn primordium stage. Plant genotypes were miR172a/Cly1.a (A–D) and Ds-miR172a/Cly1.a (E–H). Spike RNAs were hybridized with miR172a (A, E), miR172b (B, F), scrambled miRNA (negative control) (C, G) and cly1 (D, H). Cs, central spikelet; Ls, lateral spikelet; Lo, lodicule; Ra, rachis. Scale bars = 0.2 mm.
