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. 2018 May 22;122(2):251–265. doi: 10.1093/aob/mcy058

Fig. 8.

Fig. 8.

Expression of Q in immature barley spikes. (A) Localization of Q mRNA in an immature spike sampled at the awn primordium stage of WT (miR172a/Cly1) and Ds (Ds-miR172a/Cly1), as detected by in situ hybridization with an anti-sense and sense Q (3′-UTR) transcript. Scale bars = 0.2 mm in horizontal sections and 1 mm in longitudinal sections. (B) qRT–PCR-derived transcript abundance of Q during spike development in WT (miR172a/Cly1) and Ds (Ds-miR172a/Cly1). Values are mean and s.e. (n = 3 biological replications). LP, lemma primordium stage; SP, stamen primordium stage; AP, awn primordium stage; WA, white anther stage; GA, green anther stage. ns, means are not significantly different at the 5 % probability level.